In order to get it dilution was only used to dilutions 10-1, 10-2, 10-3 in the analyses of the samples. It was used methodology proposal for Hisses et al. (2001) that it was the technique of the multiple pipes for the most likely number (NMP/g). 2,3 2.3.1 MICROBIOLOGICAL DETERMINATION Determination of the Most likely Number of Coliformes Total (NMP/g) For the determination of Coliformes total used it technique of multiple pipes for the most likely number (NMP/g). It made it dilution 10-1, homogeneizando 25g and cheese with 225mL of peptonada water 1% and the seriadas dilutions (10-2, 10-3), sowed 1mL of each dilution, in third copy.

The used way was Caldo Lauril Sulfato Triptose (LST) and incubou it 35C during 24 and 48 hours. From the reading of the combination it enters the positive pipes (with growth and production of gas in the pipes of Durham) and negatives was determined the NMP/g for total coliformes. It became confirmation with Green Broth Shining Bile 2% in the same conditions of time and temperature praised by the American Public Health Association (APHA, 1992). 2.3.2 Determination of the Most likely Number of Coliformes Termotolerantes technique of the multiple pipes had been quantified using it, with Escherichia Broth coli (EC). Of total the positive pipes for Coliformes, the repicagens of the culture had become, for pipes with Broth EC. After the sowing incubou it 44,5oC for 24? 48 hours, in bath? Maria, with series of three pipes for each dilution praised for the American Public Health Association (APHA, 1992).

From the reading of the combination it enters the positive pipes (with growth and production of gas in the pipes of Durham) and negatives was determined the NMP/g for termotolerantes coliformes.